Negatively Charged, Dye Labeled-Dideoxynucleotides for “Direct-Load” DNA Sequencing
2003
Abstract A four-color set of negatively charged, single dye as well as energy transfer dye labeled-ddNTPs were synthesized and evaluated in combination with a novel polymerase in a “direct-load” DNA sequencing, obviating the laborious and time consuming post-reaction work-up.
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