Oncogenic activation of FAK drives apoptosis suppression in a 3D-culture model of breast cancer initiation
2016
// Scott Walker 1 , Fiona Foster 1 , Amber Wood 1 , Thomas Owens 1 , Keith Brennan 1 , Charles H. Streuli 1 , Andrew P. Gilmore 1 1 Wellcome Trust Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK Correspondence to: Andrew P. Gilmore, email: agilmore@manchester.ac.uk Keywords: Focal adhesion kinase, breast cancer, apoptosis, anoikis Received: September 29, 2015 Accepted: August 08, 2016 Published: September 06, 2016 ABSTRACT A key hallmark of cancer cells is the loss of positional control over growth and survival. Focal adhesion kinase (FAK) is a tyrosine kinase localised at sites of integrin-mediated cell adhesion to the extracellular matrix. FAK controls a number of adhesion-dependent cellular functions, including migration, proliferation and survival. Although FAK is overexpressed and activated in metastatic tumours, where it promotes invasion, it can also be elevated in cancers that have yet to become invasive. The contribution of FAK to the early stages of tumourigenesis is not known. We have examined the effect of activating FAK in non-transformed mammary epithelial cells (MECs) to understand its role in tumour initiation. In agreement with previous studies, we find FAK activation in 2D-culture promotes proliferation, migration, and epithelial-to-mesenchymal transition. However in 3D-cultures that better resemble normal tissue morphology, mammary cells largely respond to FAK activation via suppression of apoptosis, promoting aberrant acinar morphogenesis. This is an acquired function of FAK, because endogenous FAK signalling is not required for normal morphogenesis in 3D-culture or in vivo . Thus, FAK activation may facilitate tumour initiation by causing resistance to apoptosis. We suggest that aberrant FAK activation in breast epithelia is dependent upon the tissue context in which it occurs.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
32
References
16
Citations
NaN
KQI