Five new eremophilane derivatives from Ligularia sagitta

Abstract Ligulasagitins A–E ( 1 – 5 ), five new eremophilane-derived metabolites possibly formed via a Diels–Alder reaction in the biosynthetic process, were isolated from Ligularia sagitta Maxim. Among them 1 and 2 possess a novel C19 skeleton, 4 and 5 are two novel dimeric eremophilane type derivatives. Their structures were determined by extensive spectroscopic analysis and the structure of 2 was also confirmed by a single-crystal X-ray diffraction analysis. Furthermore, 4 and 5 showed weak cytotoxic activity against HL-60 (human promyelocytic leukemia) cells.