The effects of synthetic protease inhibitors on human proinsulin production by recombinantBacillus subtilis strain
1990
Two inexpensive inhibitors non-toxic to B. subtilis cells, O,O-diethyl-1-(N-α-hydrohexafluoro-isobutyryl)amino-1-methyl-propylphosphonate and O,O-diisobutyl-1-[2-(ethoxycarbonyl)amino-perfluoroprop-2-yl]-1-methylpropylphosphonate, when added to the culture broth, are able to prevent the proteolytic degradation of recombinant human proinsulin, secreted byB. subtilis AJ73apr73 npr− cells.
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