Effects of lead (Pb) in stormwater runoff on the microbial characteristics and organics removal in bioretention systems

Abstract Bioretention systems have been proved to be a natural approach for effectively reducing stormwater runoff pollution loads. However, the effects of heavy metals in stormwater runoff on microbial characteristics and organics removal in bioretention systems are unclear. In this study, two lab scale bioretention columns including the control and lead (Pb) treatment with the soil and filler layer were established. The changes of organic matter and lead in the effluent water and the soil (or fillers) were monitored during 121 operation days. The soil (or fillers) microbial characteristics were also analyzed. The results showed that most of Pb was intercepted by soil, while a small amount accumulated in fillers after 121 days. The long-term Pb accumulation in the biorentention system negatively affected the microbial biomass and microbial activity, while positively affected the community diversity. Pb accumulation killed some microorganisms, but simultaneously stimulated the growth of some Pb-tolerance microorganisms. The abundance of bacteria with COD degradation function in soil layer decreased, while that in fillers increased, indicating the effect of Pb on the community structure of these two layers was different. The COD removal in the soil and filler layer was promoted and inhibited by Pb contamination respectively. Moreover, Pb affected the removal of organic matter by chelating organic matters and changing their composition. The results suggested that the long-term accumulation of heavy metals in bioretention system would affect microbial degradation function and pollutants removal, causing our concern for the long-term maintenance of the bioretention system.