Grafting of encapsulated genetically modified cells secreting GDNF into the striatum of parkinsonian model rats
2001
: In order to deliver glial cell line-derived neurotrophic factor (GDNF) into the brain, we have established a cell line that produces GDNF in a continuous fashion by genetic engineering. These cells were encapsulated and grafted into parkinsonian model rats that had received unilateral intrastriatal injection of 6-hydroxydopamine 2 weeks earlier. Neurochemical analysis showed that GDNF has been produced from the capsule for 6 months after grafting and histological analysis revealed good survival of GDNF-producing cells in the capsule 6 months after grafting. The density of nigrostriatal dopaminergic fibers in the striatum as well as the number of dopaminergic cell bodies in the substantia nigra recovered significantly after GDNF-producing cell grafting. These results suggest the possible application of GDNF-producing cell grafting for the treatment of Parkinson's disease.
Keywords:
- Grafting
- Genetically modified organism
- Neuroscience
- Neural transplantation
- Parkinson's disease
- Immunology
- Encapsulation (computer programming)
- Pharmacology
- Glial cell line-derived neurotrophic factor
- Diabetes mellitus
- Biology
- Striatum
- Endocrinology
- Neurotrophic factors
- Cell
- Dopaminergic Cell
- Substantia nigra
- Internal medicine
- Dopaminergic
- Correction
- Source
- Cite
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