Decreased Programmed Death-1 Expression on the T Cells of Patients With Ankylosing Spondylitis

Abstract Background Programmed death-1 (PD-1) plays a vital role in down-modulating immune responses and maintaining peripheral tolerance. Methods The authors have investigated the inducible expression of PD-1 on activated T cells from patients with ankylosing spondylitis (As). Thirty patients with As and 31 unrelated healthy controls (HCs) were recruited in this study. The expression of PD-1 on T cells harvested from nonstimulated (t 0 ) or stimulated cultures with phytohemagglutinin for 24 hours (t 24 ) was determined by flow cytometry. The multiple levels of the PD-1 expression on stimulated and nonstimulated cells from each individual's sample (t 24 /t 0 ) represented as the degree of the inducible effect on PD-1 expression. Results The expression of PD-1 on nonstimulated T cells presented no significant difference between AS group and HC group ( P > 0.05). After stimulation, the degree of effect on PD-1 expression of CD4 + , CD4 + CD25 + , CD4+CD25 high , CD4+CD25 low and CD4 + CD25 2 T cells were significantly lower in patients with AS than those in HC group (1.9 ± 0.9 versus 3.6 ± 2.3, 9.7 ± 7.4 versus 17.8 ± 12.6, 87.8 ± 48.6 versus 157.3 ± 117.0,3.7 ± 1.4 versus 7.3 ± 2.4, 0.5 ± 0.3 versus 1.1 ± 0.6, respectively, P P > 0.05). Conclusions The decreased inducible expression of PD-1 on active T lymphocytes, especially on CD4 + CD25 high and CD4 + CD25 + T cells, may be one of important factors involved in the development of AS.