Expression of Cyclin E/Cdk2/p27Kip1 in Growth Hormone Adenomas

Objective The present study was conducted to evaluate the levels of Cdk2, cyclin E, p21, and p27 in growth hormone adenomas (GHPAs) and analyze their association with clinicopathologic features. Methods We collected 46 GHPA specimens and clinical materials from March 2012 to December 2015 at Beijing Tiantan Hospital. We analyzed the expression of Cdk2, cyclin E, p21, and p27 using immunohistochemistry, quantitative real-time polymerase chain reaction, Western blot, and methylation-specific polymerase chain reaction and sequencing. Results The levels of cyclin E and Cdk2 were much greater in the invasive group than in the noninvasive group ( P P P P P P 50%. Statistically significant differences were found in 4 CpG sites between the invasive and noninvasive specimens ( P Conclusions Overexpression of cyclin E/Cdk2 and loss of p27 appears to be associated with a poor prognosis and might play a role in the treatment of GHPAs in the future.