Establishing the dog model by blocking the end of abdominal aorta with extracorporeal approach and its significance

Objective The study was designed to investigate the feasibility of establishing the dog model by blocking the end of abdominal aorta with extracorporeal approach so as to provide a research approach for temporally controlling the arterial hemorrhoea caused by fractures on the pelvis and the sites below the pelvis in emergency situation.Methods Tracheal intubation was performed after anesthetization and fore limb was cannulated for physical multifunction monitoring in five hybrid dogs.The extracorporeal appliance made by ourselves was used to press the distal of aorta abdominalis to the centrum until completely blocking the arterial blood flow,which was judged by whether the pulse of arteria cruralis and arteriola of lower limbs was disappeared.The pressure of complete blocking of the flow of aorta abdominalis was recorded.The physical signs changes of the dogs with HR,RR,CVP,SBP,DBP,MAP were observed.Also,the dogs' condition of survival and posterior limbs' movement after palinesthesia from the anesthesia was recorded.Results Pressure of blocking the end of abdominal aorta with extracorporeal approach was(429.90±47.32)mmHg.Time for single blockage was 30 minutes.The physical signs of HR,RR,SBP,DBP,MAP,CVP kept stable before and after blood flow blockage.All of these physical signs revealed slight elevation after blood flow blockage.However,when restoring the blood flow,physical signs of SBP,DBP,MAP,HR gradually returned to the level of pre-blockage and remained stable.CVP showed initially slight decrease.And then,30 minutes after stopping blocking blood flow,CVP returned to the level of pre-blockage and maintained stable.But the RR level was still higher than that of the pre-blockage.There was no death in the experimental dogs.Meanwhile,no abnormal manifestation was found 1 week after experiment.Conclusion The dog model of blocking the end of abdominal aorta by extracorporeal approach can be successfully established,providing a mean to investigate the effectiveness of hemostasis and pathophysiologic variation in temporally blocking the end of abdominal aorta by extracorporeal approach.