Bile salts promote ToxR regulon activation during growth under virulence inducing conditions.

Cholera is an epidemic disease caused by the Gram-negative bacterium Vibrio cholerae. V. cholerae is found in aquatic ecosystems and infects people through the consumption of V. cholerae contaminated food or water. Following ingestion, V. cholerae responds to host cues to activate the expression of critical virulence genes that are under control of a hierarchical regulatory system called the ToxR regulon. The ToxR regulon is tightly regulated and is only expressed in vitro under special growth conditions referred to as AKI conditions. AKI conditions have been instrumental in elucidating V. cholerae virulence regulation, but the chemical cues within AKI medium that activate virulence gene expression are unknown. In this study, we fractionated AKI medium on a reverse phase chromatography column (RPCC) and showed that the virulence activating molecules were retained on the RPCC column, and recovered in the eluate. LC-HRMS analysis of the eluate revealed the presence of a known ToxR regulon activator, taurocholate, and other bile salts. The RPCC eluate activated the ToxR regulon when added to non-inducing media and promoted TcpP dimerization in a two-hybrid system, consistent with taurocholate being responsible for the virulence-inducing activity of AKI medium. Additional experiments using purified bile salts showed that ToxR regulon was preferentially activated in response to primary bile acids. The results of this study shed light on the chemical cues involved in V. cholerae virulence activation and suggested that V. cholerae virulence genes are modulated in response to regional-specific bile acid species in the intestine.