DEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF TRIAMCINOLONE ACETONIDE FROM POLYURETHANEINTRAOCULAR IMPLANTS

An HPLC method was developed and validated aiming to quantify the triamcinolone acetonide incorporated into intraocular implants based on polyurethaneand in direct contact with the degradation products of this biomaterial. The separation was carried out in less than 5 minutes using acetonitrile/water (50:50) as mobile phase, a C18 column and ultraviolet detection at 240nm. The method provided selectivity based on resolution among peaks. It was linear over the range of 4.0-28.0µg/mL. The quantitation and detection limits were 1.0 and 2.0µg/mL, respectively. The average accuracies of three concentrations ranged from 98.20 to 101.34% and precision was close to 2%. The proposed HPLC method can be successfully applied for triamcinolone acetonide assay incorporated into implants based on polyurethanes without any interference.