DESCRIPTIONS OF ALPHA AMYLASE PRODUCED FROM ASPEGILLUS NIGER

Alpha amylase is one of the most significant enzymes in modern biotechnology. The aim of this research was to isolate, purify and characterize the strain Aspergillus niger and one of its enzyme like alpha amylase. For this purpose potato, corn, wheat and rice were used as substrates for the production of alpha amylase. The optimum conditions for enzyme production were standardized using various parameters such as substrate, pH and temperature. The results showed that the production of alpha amylase was highest in case of potato starch in order followed by corn, wheat and rice starches. Optimum production of enzyme was recorded at 50°C temperature and 6.0 pH during 72 to 96 hours. The maximum activity of enzyme was observed in the presence of calcium chloride as compared to other metal ions. The study suggested that potato starch could be used as a potential raw material for the alpha amylase production.