Optimization of fermentation condition for Penicillium expansum alkaline lipase production using recombinant Pichia pastoris

In this study,the recombinant Pichia pastoris engineering bacteria was used to produce alkaline lipase(the engineering bacteria was constructed by our lab previously),and expression condition was improved.At first single factor experiments were performed to select factors significant influence at expression,four factors was selected:inducing time,initialize pH,methanol concentration and inoculate.Four-liner regression with orthogonal design methods were constructed based on the single factor experiments,date was tested with SPSS 18 software.The results indicated that the optimization fermentation condition was that inducing 96 h,pH6.0,0.5% methanol,inoculation 150 mL,the alkaline lipase activity was 242.7 U/mL.