Isolation and identification of NG-Monomethyl NG, NG-Dimethyl- and NG,NG-Dimethylarginine from the hydrolysate of proteins of bovine brain

Abstract N G -Monomethylarginine was isolated from the hydrolysate of a protein fraction of bovine brain. Its identification was based on elementary analysis, alkaline hydrolysis, nuclear magnetic resonance, infrared spectrum, paper electrophoresis and chromatography. N G , N G - and N G , N G -dimethylarginine were also isolated from the same source and identified. Their concentrations were determined in the protein fractions of various organs and subcellular components of brain and liver of rats.