[Effects of N-linked oligosaccharide chains on expression of integrin beta1 and apoptosis in glomerular mesangial cells].

Objective The effects of N-linked oligosaccharide chains(TM), an inhibitor of N-glycosylation of proteins, on the expression of β_1 integrin and the apoptosis of glomerular mesangial cells(GMC) were observed for exploring the role of inhibitors of N-linked oligosaccharide chains in cell apoptosis, proliferation, and expression of β_1 integrin and cyclin D_1, and hence finding a new approach to the synteresis of proliferative nephropathy. Methods Cultured rat mesangial cells were divided into 4 groups: control group, and 0.5, 1.0 and 2.0 μg/mL TM-treatment groups. The expression of β_1 integrin and apoptosis rate were measured by flow cytometry; the expression of cyclin D_1 was measured by immunohistochemistry, and proliferation of GMCs was measured by MTT. Results The expression of β_1 integrin and cyclin D_1 were decreased notably by inhibitors of N-linked oligosaccharide chains-TM. TM increased apoptosis and decreased the proliferative abilitiy of cells, and all the effects of TM were dose-dependent. Conclusion Through repressed glycosylation of glycoprotein, TM can suppress the expression of β_1 integrin, affect the adhesion of cells, and increase the apoptosis rate; at the same time, the expression of cyclin D_1 and the proliferative ability of cells were decreased, and all these were dose-dependent.