Impact of Chemical Mutagens on Cluster Bean (Cyamopsis tetragonoloba (L.) TAUB.)

In the present study the variety of cluster bean namely Ankur Rani was used to inducegenetic variability. For these study two chemical mutagens, namely EMS of differentconcentration such as 0.05%,0.10%,0.15% and SA of different concentration such as 0.01%,0.02% and 0.03% were used. The seeds of variety Ankur Rani of Cluster bean were obtainedfrom Local market of Aurangabad (Maharashtra).These seeds were subjected to mutagenesis byusing chemical mutagens. M1 generation was raised and effect of these mutagens on differentparameterslikegerminationpercentage,seedlingheight,leafmorphological changes,chlorophyll chimeras, pollen sterility, and plant survival percentage. Both the mutagens hadshown an inhibitory effect on seed germination. The 0.15% EMS showed highest germinationpercentage. The 0.03% SA showed lowest germination percentage. Seedling height was found toincrease as concentration of both the mutagens. But exceptionally it is found to be