MSMEG_6292, a Mycobacterium smegmatis RNA polymerase secondary channel‐binding protein: purification, crystallization and X‐ray diffraction analysis

The transcriptional activity of RNA polymerase (RNAP) is controlled by a diverse set of regulatory factors. A subset of these regulators modulate the activity of RNAP through its secondary channel. Gre factors reactivate stalled elongation complexes by enhancing the intrinsic cleavage activity of RNAP. In the present study, the protein MSMEG_6292, a Gre-factor homologue from Mycobacterium smegmatis, was expressed heterologously in Escherichia coli and purified using standard chromatographic techniques. The hanging-drop vapour-diffusion crystallization method yielded diffraction-quality crystals. The crystals belonged to the trigonal space group P3(1)21 (or its enantiomorph P3(2)21), with unit-cell parameters a = b = 83.15, c = 107.07 angstrom, alpha = beta = 90, gamma = 120 degrees. The crystals diffracted to better than 3.0 angstrom resolution. Molecular-replacement attempts did not yield any phasing models; hence, platinum derivatization was carried out with K2PtCl4 and derivative data were collected to 3.4 angstrom resolution.