Serratia plymithica synthetic culture medium and method for preparing fermentation liquid of serratia plymithica synthetic culture medium

2012 
The present invention relates to a method for preparing Serratia coli fermentation broth and a synthetic medium, the medium composition (g / L) as: colloidal chitin 2 to 10, 3 to 10, beef extract, peptone 5 to 15 , sodium chloride 6 to 10, 1 to 5, ammonium sulfate, sodium citrate, 1 to 3, dipotassium phosphate 1 to 5, 0.5 to 3 magnesium, ferrous sulfate 0.01 to 0.05. Yield broth inoculum of 1% to 10% in an amount of 28 ~ 35 ℃ shaker 140r / min continuous culture 4 ~ 6d, to obtain high suppressing aflatoxin active material and chitinase of Serratia marcescens. The fermentation supernatant chitinase activity of 2.04 ~ 5.36U / ml; Aspergillus parasiticus on mycelial growth inhibition rate of 80.13 - 89.56%, of the aflatoxin produced inhibition rate 90.22 - 98.31%. Medium composition of the invention is reasonable, preparation method is simple and reliable.
    • Correction
    • Source
    • Cite
    • Save
    • Machine Reading By IdeaReader
    0
    References
    0
    Citations
    NaN
    KQI
    []