Design of Epitope Based Vaccine for Dengue Virus Using Immunoinformatic Approach

Dengue is an infectious disease caused by one of the four serotypes of the dengue virus. It is a mosquito borne disease and the female Aedes mosquito is mainly spread to humans. The disease exists mostly in tropical and subtropical areas, placing about a third of the human population at risk of infection worldwide. The tremendous variety and the minimal similarity in identity at the level of amino acids contribute to a troublesome obstacle in the production of an effective vaccine. Vaccine production has been oriented towards the rational design of the epitope based vaccine through available immunological evidence, the advancement of the antigenic peptide prediction method, and the integration of molecular docking into immunoinformatics. So we are discovering new prevention steps, using immunoinformatics methods to create dengue epitope based subunit vaccine that can produce different immune responses inside the host. For structural and non-structural dengue virus proteins, distinct B and T cell binding epitopes were expected. Microscopic interactions between the epitope and the allele were confirmed by molecular docking. In this work epitopes KRSGIQEVD, RRDLRLAAN, RRGDLPVWL, FQIYKRSGI and THSSAAQRRGRIGRNP were predicted as a new immunotherapeutic agent for dengue vaccine that can provide prevention and control of outbreaks.