LincRNA metastasis-associated in lung adenocarcinoma transcript 1 expression in osteosarcoma and its function in cells migration

Objective To investigate the expression of metastasis-associated in lung adenocarcinoma transcript l (MALAT1) in human osteosarcoma tissue and cell line MG63 expression and its biological function in the proliferation and migration. Methods 23 osteosarcoma patients and human osteosarcoma cell line MG63 were selected for the study. Osteosarcoma tissue, adjacent tissues and MG63 cells were analyzed for MALAT1 relative expression by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) assay. SiR_MALAT1 was designed according to MALAT1 sequence. Lipo2000 was used to transfect small interfering RNA (siR_MALAT1), non-correlated sequence (siR_NC) and simple lipo2000 (control group, MG63_lipo2000) into MG63 cells. Cell counting kit-8 (CCK-8) test and wound healing assay were used to investigate whether the proliferation and migration ability of MG63 cells changed after MALAT1 was down-regulated. Results 18 (78.3%) were highly expressed in cancer tissues and 5 (21.7%) were low expressed in cancer tissues of the included 23 cases. The relative expression level of MALAT1 in human osteosarcoma MG63 cells was 4.02±0.88). After transfection of siR_MALAT1, siR_NC and lipo2000 alone, the expression levels of MALAT1 were 1.56±0.36, 4.12±1.03 and 3.98±0.91, respectively. SiR_MALAT1 could significantly down-regulate the expression level of MALAT1 in MG63 cells (F=15.390, P 0.05). After siR_MALAT1 down-regulated the expression of MALAT1, the migration ability of MG63 cells decreased significantly. Conclusion MALAT1 was up-regulated in osteosarcoma tissues and osteosarcoma cell line MG63, and its high expression was related to cell migration. Key words: Osteosarcoma; Metastasis-related transcript l of lung adenocarcinoma; Biological function; Invasion and metastasis