The detection method of the highly sensitive mutant gene

Quickly the presence or absence of a mutant gene that mixed in the gene pool, also conveniently and with high accuracy, and a method capable of detecting with high sensitivity, high sensitivity detection of various, especially PNA-LNA-PCR clamp method to provide an improved method. As pre-process for the main detection step, a clamp primer consisting PNA hybridizing to all or part of the target site having a sequence complementary to the sequence or wild-type gene (1) wild-type gene, (2) mutant gene a primer capable of amplifying a region containing a target site with the sequence of the region containing the (3) the the gene pool, the coexistence in a gene amplification reaction, the target site of the mutant gene by a gene amplification method implementing the pre-amplification step of selectively amplified.