COMPARATIVE STUDY OF PSEUDOMONAS AERUGINOSA ISOLATE RECOVERED FROM CLINICAL AND ENVIRONMENTAL SAMPLES AGAINST ANTIBIOTICS Research Article

Pseudomonas aeruginosa has emerged as one of the most potential problematic gram negative pathogen, the present study to investigate the prevalence of P. aeruginosa from the various environmental and clinical samples. The highest isolation rates of P. aeruginosa was found in clinical 71%, followed by industrial effluent 58.8%, soil 50%, water 45.45% and air 43.45%. The antibiotic susceptibility test was performed by the disc diffusion method according to NCCLS (National Committee for Clinical and Laboratory Standard) guidelines. The traditionally clinical pertinent antibiotics like Amikacin (Ak), Ceftazidime (Ca), Netilmicin (Nt), Gentamicin (G), Piperacillin (Pc), Ciprofloxacin (Cf), and Imipenem (I) were tested against P. aeruginosa. Among the antibiotics, the most effective antibiotic were carbapanems and aminoglycosides and the resistance rates were detected as 18% and 28%, respectively among 50 P. aeruginosa strains. Over 20% of the isolates were exhibited multi-drug resistance to five (or) more antibiotics, especially clinical isolates. In conclusion, the results indicates, the excessive use and disposing of antibiotic and chemicals leads to the emergence of antibiotic resistance in the environment and hospital. So that proper monitoring and optimization should be adopted.