Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips

Automated microscale chromatography using pipette tips packed with microliters of resin is seen as an increasingly attractive option for high-throughput screening of purification conditions during early phase biopharmaceutical development. Two types of data that can be produced by these studies are uptake curves and isotherms, providing valuable fundamental separation data to assist with the prediction of larger-scale performance. This chapter will describe an operating protocol for this type of experiment using the example of ovine polyclonal antibodies binding to a multimodal weak cation exchange resin.