[Preparation of agar-paraffin double-embedded longitudinal sections of Schistosoma japonicum].
2012
Schistosoma japonicum adults are pre-embedded in a double-layer agar and made the block,then dehydrated with alcohol,isobutyl alcohol and n-butyl alcohol.Various staining procedures can be conducted after conventional sectioning and dewaxing.Complete longitudinal serial sections of the pre-embedded worms can be obtained,and the desired sections can be easily located accurately.
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