Effect of DNA Aptamer (WD-Aptamer) on Hair Regrowth by Inhibiting Wnt Signaling Negative Regulation

CXXC5 negatively regulates the Wnt signaling pathway by binding to the Wnt signaling component protein disheveled (Dvl). The Dvl protein comprises three domains: the DIX domain, the DEP domain, and the PDZ domain. The PDZ domain binds to the CXXC5 zinc finger domain. We prevented the CXXC5 effect by using a DNA aptamer (WD-Aptamer) that binds to the Dvl1_PDZ domain. The inhibitory effect of CXXC5 was confirmed through tests of cell proliferation in HaCaT cells and in vivo experiments using mice.