Basic and Translational Science Effect of Human Muscle-derived Stem Cells on Cryoinjured Mouse Bladder Contractility

The contractile powers of bladder muscle strip in the C group were more decreased than the N group after EFS, Ach, and ATP treatment (P .05). The bladder contractility of the M group was more increased than in the C group (P .05), but was lower than the N group after EFS and Ach treatment. However, there was no significant difference of contractile power between the C and M groups after ATP stimulation. In immunohistochemical staining, the thickness of the bladder smooth muscle layer in the M group was significantly increased compared with the C group, and PKH26-labeled implanted cells were positive for smooth muscle cell differentiation marker (-SMA) in the injected region. CONCLUSION hMDSCs injection increased cholinergic bladder contractile power but not the purinergic component of bladder contraction after cryoinjury. UROLOGY 80: 224.e7–224.e11, 2012. © 2012 Elsevier Inc.