Analysis of sesterterpenoids from Aspergillus terreus using ESI-QTOF and ESI-IT.

Introduction - Biosynthesis of terretonin was studied due to the interesting skeleton of this series of sesterterpenoids. Very recently, Lopez-Gresa reported two new sesterterpenoids (terretonins E and F) which are inhibitors of the mammalian mitochondrial respiratory chain. Mass spectrometry (MS), especially tandem mass spectrometry, has been one of the most important physicochemical methods for the identification of trace natural products due to it rapidity, sensitivity and low levels of sample consumption. The potential application prospect and unique skeleton prompted us to study structural characterisation using MS. Objective - To obtain sufficient information for rapid structural elucidation of this class of compounds using MS. Methodology - The elemental composition of the product ions was confirmed by low-energy ESI-CID-QTOF-MS/MS analyses. The fragmentation pathways were postulated on the basis of ESI-QTOF-MS/MS/MS and ESI-IT-MSn spectra. Common features and major differences between ESI-QTOF-MS/MS and IT-MSn spectra were compared. For ESI-QTOF-MS/MS/MS experiments, capillary exit voltage was raised to induce in-source dissociation. Ammonium acetate or acetic acid were added into solutions to improve the intensity of [M + H](+). The collision energy was optimised to achieve sufficient fragmentation. Some fragmentation pathways were unambiguously proposed by the variety of abundance of fragment ions at different collision energies even without MSn spectra. Results - Fragmentation pathways of five representative sesterterpenoids were elucidated using ESI-QTOF-MS/MS/MS and ESI-IT-MSn in both positive- and negative-ion mode. The key group of characterising fragmentation profiles was ring B, and these fragmentation patterns are helpful to identify different types of sestertepenoids. Conclusion - Complementary information obtained from fragmentation experiments of [M + F](+) (or [M + NH4](+)) and [M - H](-) precursor ions is especially valuable for rapid identification of this kind of sesterterpenoid. Copyright (c) 2010 John Wiley & Sons, Ltd.