[Analysis of variant translocation der ins (17; 15) in patient with APL by G-banding technique and interphase fluorescence in situ hybridization].

To investigate the biological characteristies of the variant translocation der ins(17;15) in a patient with acute promyelocytic leukemia (APL),the conventional G-banding technique,interphase fluorescence in situ hybridization (int-FISH),RT-PCR,gene scaning,gene sequence and flow cytometry were performed. The results indicated that the variant translocation der ins (17,15) observed by G banding technique was a rare type,the int-FISH assay by using dual-color pml/rarα fusion probes confirmed the cytogenetic findings. The detection results of other molecular methods demonstrated the existence of the whole pml/rarα fusion gene,while this case had insertion variant translocation. This patient got complete remission by using combined chemotherapy,and survives with continuous complete remission during following up for 1 year. In conclusion,the variant translocation der ins(17;15) is rare type in APL,its incidence is lower,several signal types in detection of int-FISH were observed and the combination chemotherapy for this patient showed more obvious efficacy.