Two-photon image-based synapse detection method

The invention discloses a two-photon image-based synapse detection method, and relates to the technical field of mode identification and neurology. The method comprises the steps of obtaining a two-photon image; dividing the two-photon image into an axon image and a dendrite image; performing 2D detection of axon noduli in the axon image; performing 2D detection of dendrite spines in the dendrite image; performing 2D synapse detection by using the detected axon noduli and dendrite spines; and performing 3D synapse detection based on a 2D synapse detection result. Through the method, the synapse detection efficiency and precision are improved; and the method has universality and is not sensitive to image types.