Quality control study of borneol and artificial musk in Xinfufang-Zhenzhusan and Xinfufang-Zhenzhugao

Objective To establish a quality control method for borneol and artificial musk in Xinfufang-Zhenzhusan and Xinfufang-Zhenzhugao. Methods We used petroleum ether - toluene - ethyl acetate (9:3:2)as developer for TLC to identify isoborneol and borneol and petroleum ether-dichloromethane (2:3) as developer for TLC to identificate musk ketone. Agilent 7890 B gas chromatograph, FDI detector; Column: Thermo-TG-WaxMS GC (0.25 mm × 30 m, 0.25 mm) was employed; the carrier gas was high purity nitrogen and flow rate for 1 mg/ml, the injection port temperature is 200 ℃ and detector temperature is 250 ℃; the split ratio is 10:1 and injection volume was 1 μl, using temperature programmed. Results The isoborneol, borneol and musk ketone in the range of 0.001-10 mg/ml showed good linearity. The recovery of the method is in the range of 95 % to 105 %. The TLC for isoborneol, borneol, musk ketone can be identified easily. Conclusions The method was simple and reasonable, which can be used for the quality control of borneol and artificial musk in the Xinfufang-Zhenzhusan and Xinfufang-Zhenzhugao. Key words: Chromatography, thin layer; Chromatography, Gas; Borneolum syntheticum; Artificial musk; Isoborneol; Borneol; Muscone; Quality control