Effect of microRNA-10b silencing on expression of peroxisome proliferator activated receptor-a in human pancreatic cancer cell line

Objective To observe the effect of silencing microRNA-10b on the expression of peroxisome proliferator activated receptor-α(PPAR-α) in human pancreatic cancer cell line AsPC-1.Methods The recombinant eukaryotic expression plasmid vector with miR-10b antisense sequence was transfected into AsPC-1 cells with lipofectamine,and the efficiency of the instant expression was confirmed by real-time quantitative polymerase chain reaction (Real-time PCR) and fluorescence microscope.The difference of the proliferation of the cells was measured by proliferation assay,and the expression of PPAR-α was detected by Western blotting.Results The expression of miR-10b in the antisense groups was decreased to 14％ as compared with the control group (P ＜ 0.05).The cell proliferation in the antisense groups was decreased to 36％ as compared with the control group (P ＜ 0.05).The expression of PPAR-α protein in the antisense groups was 1 230.5 ± 110.4,and that in the control groups was 2 398.1 ± 156.3 and 2 603.7 ± 227.5 (P ＜0.05).The apoptosis ratio in the antisense group was 19.4％,and that in the control groups was 10.1％ and 11.5 ％ (P ＜ 0.05).Conclusion The silencing of miR-10b can inhibit the expression of PPAR-α and the cell proliferation,and promote the apoptosis of the ASPC-1 cells. Key words: Pancreatic cancer;  MicroRNA-10b;  Peroxisome proliferator activated receptor-α