Immunohistochemical Localization of Endocrine G-Cells in the Epithelium of the Pars pylorica Mucosa of the Cat and Mouse Stomach

Cerny H., S. Mazanek, Eva Cerna: Immunohistochemical Localization of Endo­ crine G-Cells in the Epithelium of the Pars pylorica Mucosa of the Cat and Mouse Stomach. Acta vet. Bmo, 60,1991: 317-322. Tissue samples obtained from the pyloric part (antrum pyloricum) of the sto­ mach of 6 mice and 4 adult cats were fixed in Bouin's solution, embedded in para­ plast and sectioned at 5 to·7 pm. Endocrine G-Cells were demonstrated in the sections immunohistochemically using a MILAB-ICC kit for immunohistochemical demonstration of gastrin. The final colour visualization was achieved by the binding of the PAP complex. The study of the distribution, cytomorphology and G-cell types in the epithe­ lium of the mucosa and glands yielded the following results: 1. Endocrine G-cells were scattered diffusely in the basal part of the mucosa. 2, In the cat they occupied the lower half of the "height of the mucosal epi­ thelium, in the mouse they were bound topographically to the basal quarter or fifth of the total height of the mucosal epithelium. 3. In both species the surface cells were immunopositive; positive reactions were observed locally also in non-cellular structures of the mucosal surface. 4. In both species two types (open type and closed type) of endocrine G-cells were found to occur. 5. In both cats and mice open-type G-cells were fewer in number than closed­ -type cells. 6. Cells with polymorphic cytoplasmic projections were observed in both species, but more frequently in· the mouse than in the cat. Gastric mucosa, pars pylorica, G-cell, immunohistochemistry, cat, mouse