Effects of caffeine on shuttle, operating behaviors, and brain metabolism in rats

Effects of caffeine (30, 60, and 120 mg.kg-1, ip) on shuttling and operating behaviors in 80 rats were studied. Effects of caffeine (60, 120 mg.kg-1, ip) on contents of dinucleotides and pterins in 6 brain areas of 18 rats were investigated by HPLC with fluorescent detector. The results showed that the dose of caffeine that induced augment of shuttle behavior was lower than that induced operating behavior. Caffeine 120 mg.kg-1 inhibited both shuttling and operating behavior, decreased FAD content in caudate nucleus; caffeine 60 mg.kg-1 increased FAD in cerebellum and brain stem. Caffeine 60, 120 mg.kg-1 increased brain NADH contents, decreased pterin contents, and increased biopterin contents in some brain areas.