Plant Regeneration Through Tissue Culture Of Millet Pear ( Pennisetum Glaucum (L) R.)

Many problems were missed with immature embryos and shoot explants pieces used; principally: Calli were initiated in plant flowering period (every 110 days), and the low percentage of embrogenesis what is the source used. So for Pennisetum Glaucumnew embryogenic calli sources were explored from calli initiation to fertile plant regeneration. About 90% of dissected apices shoot, seedlings and seeds developed calli. From meristems position, 6 mm of shoot and 20 mm of root developed 50 to 100% of calli. No embryogenic calli were obtained from root. More than 80% of dissected apices led to embryogenic calli. Maintained on MS (1.1, 2.5), MS (5) and N6 (1.100.25) media culture, calli embryogenic potential and fertile plant regeneration were conserved for more than 12 months. Characteristics of regenerated plants were similar to control. It appears that it has been shot in a suitable way. The difficulties associated with the use of the immature embryo and stem explants in tissue cultures are numerous; including cal initiation, which occurs only during flowering, for the immature embryo, every 110 days, and the low percentage of embryogenesis. For Pennisetum Glaucumnew sources of embryogenic calli have been explored from callus initiation to fertile plants. About 90% of apical meristems, sprouts and grains developed callus. From the meristems, only 6 mm of stem and 20 mm of root developed calluses with a rate of 50 to 100%. The root has developed no embryogenic callus. More than 80% of apical meristem calli were embryogenic. Calli maintained on MS (1.1.2.5), MS (5) and N6 (1.100.25) remained embryogenic and produced fertile plants for at least 12 months. Regenerated plants have similar characteristics to control plants. It appears that the apical meristem is a new material suitable for tissue culture