Development of Microbial Nanofactory for Zinc, Magnesium, and Titanium Nanoparticles Production Using Soil Fungi

To access the nanoparticle production potential over a hundreds of fungi were isolated from the soil and tested with precursor salts of Zn, Mg and Ti. Initially, the nanoparticle size was calculated by using the dynamic light scattering through particle size analyzer. Only 14 fungi isolate were found suitable for nanoparticle biosynthesis. All the fourteen fungal isolate identified as molecular level, out of which six were identified as Aspergillus flavus, two each as Aspergillus terreus and Aspergillus tubingensis and one each as Aspergillus niger, Rhizoctonia bataticola, Aspergillus fumigatus, and Aspergillus oryzae. To understand the reason of variable potential of nanoparticle production, extracellular protein content was measured. The results suggested that CZR 1 isolate of Aspergillus terreus showing the maximum 1480.98 gm L −1 extracellular protein contents and have potential for nanoparticle synthesis of all the three Zn, Mg and Ti metals. The 32 kDa protein was responsible for the synthesis of Zn, Mg and Ti nanoparticles from its precursor compound ZnO, MgO and TiO2 respectively.