Solid phase extraction of acrolein 2,4-dinitrophenylhydrazone for HPLC analysis

Abstract Solid phase extraction (SPE) was employed for the separation of the acrolein 2,4-dinitrophenylhydrazone from strong acids for HPLC analysis. Analysis speed, precision and simplicity were increased over conventional liquid-liquid extractions and samples could be stored on SPE columns for at least 2 weeks before HPLC analysis. The precision was ±3% over the range 0.1–16 μg ml −1 and the quantitation limit was 0.04 μg ml −1 , defined as four times the variance of the blank.