Analysis on the HPLC chromatographic fingerprint of gegen qinlian decoction

Objective: To investigate the multiple bioactive constituents in Gegen Qinlian decoction and evaluate its bioactivity and quality control.Methods: A high performance liquid chromatography with diode array detection(HPLC-DAD)method for the simultaneous qualitative determination of multiple components was developed.The separation was performed on a Kromasil C18 column by gradient elution with acetonitrile and ammonium acetate(containing 0.3%triethylamine,and adjusted pH 4.3 using 1% glacial acetic acid)as the mobile phase at a flow-rate of 0.7 mL/min,the column temperature was maintained at 30℃,HPLC chromatographic comparison between Gegen Qinlian decoction,four medicinal material and 14 components were carried out to investigate components in the composite formula.Results: 23 characteristic peaks were identified and could be dated back to four medicinal materials from Gegen Qinlian decoction,in which 12 peaks were identifed by standards.Conclusion: This readily available,low-cost and reliable HPLC-DAD method can be used for simultaneous analysis of multiple bioactive constituents of Gegen Qinlian decoction and will improve the quality control of Gegen Qinlian decoction.