PKG pathway is involved in the Spermine-NONOate induced acrosome reaction in buffalo spermatozoa

Acrosome reaction is very important reaction which occurs in capacitated spermatozoa and involves the fusion of outer acrosomal membrane with overlying plasma membrane and release of mainly acid hydrolases and renders the potential of fertilization in spermatozoa. Spermine-NONOate a nitric oxide donor also causes the AR through the synthesis of cGMP and activation of protein kinase G (PKG). In this study there is protein tyrosine phosphorylation of p20.p32, p45, p49, p69, p80 and p105 proteins. Spermine-NONOate in presence of PKG inhibitor resulted in significant inhinbitoon of AR as compared to spermine-NONOate alone (