“Pop-slide” patterning: rapid fabrication of microstructured PDMS gasket slides for biological applications

We describe a “pop-slide” patterning approach to easily produce thin film microstructures on the surface of glass with varying feature sizes (3 μm to 250 μm) and aspect ratios (0.066–3) within 45 minutes. This low-cost method does not require specialized equipment to produce microstructured gasket layers for sandwich assays and could be readily applied to many biological applications.