Unusual Diversity of Acquired β-lactamases in Multidrug-Resistant Pseudomonas aeruginosa Isolates in a Mexican Hospital

Aims: To investigate the presence of extended spectrum and metallo β-lactamases (MBLs) in Pseudomonas aeruginosa isolates which are resistant to imipenem and ceftazidime that were isolated in a hospital in Mexico. Results: Pulsed-field gel electrophoresis (PFGE) revealed the presence of four clonal types among the 14 isolates. All these genes were found either alone or simultaneously in the P. aeruginosa strains in the following five different arrangements: ; ; ; ; and . Class 1 integrons were detected and contained the cassettes blaGES-5 and blaOXA-2, but not that of blaVIM. blaVIM genes occurred only in the chromosome, while blaGES-5 was located in the chromosome and in the plasmids. Conclusions: To our knowledge, this is the first description of P. aeruginosa strains simultaneously producing the VIM-2 and VIM-11 variants, and the combination of GES-5 and MBL carbapenemases, whi...