Study of triplet state of the primary electron donor on preparations of reaction centers of photosynthetic bacteria using magnetooptical absorbance difference spectroscopy

The authors use magneto-optical absorbance difference spectroscopy based on fluorescence determination to investigate the photosynthetic membrane reaction centers of two photosynthetic bacteria, the blue-green mutant of Rps. sphaeroides R-26 and the cytoplasmatic membrane from C. aurantiacus, and their primary electron donors. The mechanism of triplet state excitation under the action of continuous irradiation and a modulating magnetic field is described in detail. Data are also given on hyperfine structure determined by computer processing of the spectral image.