Characterization of cDNA clone coding for nodulin-45 from yellow lupin (lupinus luteus)

Abstract We describe in this paper a characterization of full-length (1396 bp) nodule-specific cDNA clone (pLN-13) from yellow lupin root nodules. The mRNA corresponding to the cDNA insert appears prior to the onset of nitrogenase activity, i.e. 11 days after infection. Hybrid-released translation analysis revealed that the selected mRNA encodes a single polypeptide of molecular weight 45 000. According to the nomenclature proposed for nodulins, this protein was named nodulin-45. The primary sequence and deduced amino acid composition indicates that nodulin-45 is a highly hydrophilic protein with potential N-terminal signal peptide and two putative asparagine-linked glycosylation sites.