Optimization of fermentation conditions for enhanced glutamic acid production by a strain of Corynebacterium glutamicum NIAB BNS-14.

Corynebacterium glutamicum NIAB BNS-14 was used for enhancing glutamic acid production for which eight different carbon (glucose, fructose, sucrose, maltose, lactose, dextrin, sorbose, and galactose) and seven nitrogen sources (ammonium chloride, ammonium sulphate, ammonium nitrate, ammonium phosphate, urea, potassium nitrate and sodium nitrate) alongwith various concentrations of biotin (0.5, 1.0, 2.5, 5.0, 10, 15µg/ml) were used. The strain gave maximum production as 8.5g/l of glutamic acid at 10% fructose and 3% ammonium nitrate with 10µg/ml biotin at 30°C and 150rpm after 72hrs of fermentation. The cell mass obtained was 21.1g/l.