Comparison of platelet quality in washed platelet concentrates prepared with different synthetics storage solutions.

Washed platelet concentrates (WPC) have been used for patients with severe non-hemolytic febrile transfusion reaction (NHFTR) following ordinary PC transfusion. We studied three synthetic platelet storage solutions, namely Solution 1 containing saline including 0.1% citrate, Solution 2 based on acetate Ringer's solution with glucose and citrate, and Solution 3 being Seto solution designed for longer storage of PC. The pH of PC washed with Solution 1 rapidly decreased from 6.37 to 5.82 after 1 day storage, but remained at 6.8 throughout storage for 5 days with Solutions 2 and 3. On day 5, Pselectin concentrations in the supernatant of PC washed with Solution 3 were still lower than those washed with Solution 1 or Solution 2 and stored for 1 day or 3 days, respectively. The percent hypotonic shock response (%HSR) of Solution 1 was completely lost after 1 day storage, and Solution 2 reached about 20% after 3 day storage. %HSR and discoid shape were maintained at a high level, for 5 days by using Solution 3. These results suggest that Solution 3 is the most desirable solution for platelets. Owing to the rapid deterioration of platelet quality, we recommended that transfusion of WPC prepared with Solution 1 or Solution 2 be done within 6 hr or 1 day, respectively.