Study of protein structure in insect virus (PrGV) by laser Raman spectroscopy

We report here our observation of Raman spectrum of the Granulosis Virus of cabbage butterfly Preris rapae (PrGV) and investigate the protein structure of the inclusion body of PrGV. The intense Amide III line at 1248 cm-1 and Amide I line at 1668 cm-1 in the Raman spectrum of PrGV contains a predominantly random-Coil or (beta) -turn secondary structure. The intensities ratio of the tyrosyl lines 850 cm-1 and 830 cm-1 was found to be 1.25, it indicates the tyrosyl residues are exposed to a solvent. According to s-s stretching vibration frequency 509 cm-1, the protein molecules of PrGV have gauche-gauche-gauche linkage. The strong peak at 1367 cm-1 is typical of Tryptophans buried in a hydrophobic environment.© (1993) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.