Effects of bone marrow mesenchymal stem cells on pancreatic stellate cells of rats with chronic pancreatitis

Objective To observe the effects of pancreatic stellate cells (PSC) of rats with chronic pancreatitis (CP) after injection of the bone marrow mesenchymal stem cells (BMSCs).Methods The 80 SD rats were randomly divided into four groups:control group (20 rats were injected with sterile saline),model group [20 rats were injected with dibutyltin dichloride (DBT) through the tail vein to make CP model ],treatment group (20 rats were injected with allogeneic GFP-MSCs cultured in vitro when the model of CP was established),and sham treatment group (20 rats were injected with equivalent sterile saline when the model of CP was established).The expression of desmin,glial fibrillary acidic protein (GFAP),α-smooth muscle actin (α-SMA),collagen type Ⅰ and Ⅲ,TGF-β1,IL-10 and TNF-α was detected in the rat pancreas of each group.Results In treatment group,the activity of collegen type Ⅰ (0.135±0.030) ng/g,Ⅲ (0.029±0.008) ng/g,TGF-β1 (0.020±0.006) μg/L in PSC was lower than in sham treatment group ( P ＜ 0.05 ),while that of IL-10 (603.799 ± 89.374) g/ml and TNF-α(507.45 ±90.13) μg/L was higher.All of them had no statistically significant difference between sham treatment group and model group ( P ＞ 0.05 ).No obvious exception was observed in control group.Conclusion By controlling cytokines,BMSCs could reduce the activation,proliferation and differentiation of PSC and lower the deposition of extracellular matrix. Key words: Bone marrow mesenchymal stem cells; Chronic pancreatitis; Stellate cells