Auxin regulation in the interaction between Rice stripe virus and rice

The expression of YUCAA1 gene and the amount of endogenous IAA in rice(Oryza sativa subsp,japonica)plants and rice suspension cells infected by Rice stripe virus(RSV)were investigated by Real-time RT-PCR and high performance liquid chromatography,respectively.And the results showed that the expression of YUCAA1 gene and the amount of endogenous IAA increased at various times(16,32,48 and 64 h)after post-infection by RSV in rice suspension cells.In rice plants infected by RSV,the expression of YUCAA1 gene and the amount of endogenous IAA increased at 4～8 d after post-infection as comparison with that of healthy rice plants,and decreased at 12 and 16 d.These results indicated that RSV infection could regulate auxin biosynthesis in rice. Additionally,the expression of RSV CP increased 2.9 times in rice plants after it was treated with KPSC buffer to deplete the endogenous auxins,and decreased 45% after 30μmol/L IAA treatment.All of these results suggest that the auxin may play a role among RSV replication in rice plant.