Biological and Molecular Studies on an Egyptian Potyvirus Isolate from Hyocyamus muticus L.

ANEW isolate of potyvirus was isolated from naturally-infected……. henbane plants (Hyocyamus muticus L.) collected from thefarm of Faculty of Pharmacy, Cairo University in January 2013. Thenaturally infected henbane plants exhibited severe mosaic, rugosity,blistering and malformation. Thermal inactivation point, dilution endpoint and longevity in vitro of virus isolate were found to be 65 oC,10- 4 and 4 days, respectively. Electron micrograph of partial purifiedvirus negat ively s t ained wit h 2% p hosp hot unges t ic acidrevealed the presence of filamentous viruses with size 1440 X14.3nm.Total RNA was extracted from infected henbane plant. Comparativenucleotide sequence analysis for virus showed a high degree ofsimilarity (62 %) with four potyviruses accessions (EU482153: Potatovirus Y isolate Foggia, KF850513: Potato virus Y isolate M3,AM184113: Henbane mosaic virus partial gene for polyprotein-PHYS/H isolate, AY166867: Potato virus Y strain N isolate). Thevirus isolate was published in GenBank with accession numberKM497011.