Automated simultaneous release of intact and unreduced N and O-linked glycans from glycoproteins

Oligosaccharidesingl co roteinsmay generally be attached to eptides in two ways, gy 8-glycosidic linkage to asparagine or g 0-glycosidic linka e to serine or threonine. The structures of &can chain attachecfthrough each linkage are different and they can have different structural and functional roles. Although in the past more attention has been aid to N-linked glycans there is currently growing interest in &inked glycans. Detailed study of the structure of the lycans r uires their release from the peptide. A number of di B " i , erent met ods, both chemical and enzymatic exist. Enzymatic methods such as ycosidase F may give incomplete or selective re-