DETERMINATION OF TRIFLUSAL IN HUMAN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH AUTOMATED COLUMN SWITCHING SYSTEM

An analytical method of triflusal in human plasma was developed using semi-microbore HPLC equipped with automated column switching system. p-Toluic acid which is the structural analogue of triflusal, was used as an internal standard and 2 M HCl was employed as a stabilizer. The load phase and mobile phase were prepared using acetonitrile and 20 mM KH2PO4 with the volume ratios of 10:90 (pH 2.5) and 43:57 (pH 2.3), respectively. The signals were monitored by a UV detector at 275 nm with flow-rate of load phase, 500 μL/min, and mobile phase, 100 μL/min, respectively. The retention time of triflusal and p-toluic acid was about 20.2 min and 16.4 min, respectively. The detection limit of triflusal in human plasma was 0.01 μg/mL and the limit of quantitative analysis was 0.05 μg/mL. The accuracy of the assay was from 97.76% to 116.51% while the intra-day and inter-day coefficient of variation of the same concentration range was less than 15%. This analytical method demonstrated excellent sensitivity, reproducib...